Efecto antimicrobiano de un extracto de la raíz de Salvia munzii contra microorganismos de importancia odontológica
Palabras clave:
Salvia munzii, efecto antibacteriano, acción antifúngica, microorganismos bucales, Enterococcus faecalis, Streptococcus mitis
Resumen
El objetivo de este estudio fue evaluar el efecto antimicrobiano de un extracto de la raíz de la planta Salvia
munzii contra microorganismos de importancia odontológica. Utilizando un rotavapor Büchi R-215 se
elaboró un extracto hexánico de la raíz de Salvia munzii. Posteriormente, se probaron diferentes concentraciones
(22, 11 y 5,5 mg/ml) de este extracto frente a Enterococcus faecalis (ATCC 51575), Streptococcus
mitis (NCIMB 13770), Streptococcus mutans (ATCC 35668), Candida glabrata (ATCC 15126), y
Candida krusei (ATCC 14243). A continuación, se determinó la concentración mínima inhibitoria para
cada microorganismo utilizando diluciones subsecuentes. Para analizar los datos se utilizó el análisis de
varianza seguido por una post-prueba de Dunnett. Se consideró diferencia estadística cuando p<0.05. El
extracto de la raíz de Salvia munzii inhibió el crecimiento de todos los microorganismos utilizados en los
experimentos de este estudio. El mayor efecto antibacteriano de Salvia munzii fue de 30,67±0,66 mm
observado contra el Streptococcus mutans; mientras que la mayor acción antifúngica fue de 17,83±0,16
frente Candida glabrata. Las concentraciones mínimas inhibitorias confirmaron que el microorganismo
más sensible al extracto fue el Streptococcus mutans (0,34 mg/ml). El extracto de la raíz de Salvia munzii
mostró actividad antibacteriana y antifúngica contra todos los microorganismos de importancia clínica
evaluados en este ensayo de laboratorio mediante la técnica de difusión de pozos.
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Citas
Acquaviva R, Malfa GA, Di Giacomo C.
Plant-Based Bioactive Molecules in Improving
Health and Preventing Lifestyle Diseases. Int J
Mol Sci. 2021;22(6):2991.
Adame-Miranda SJ, Granados-Guzmán G,
Silva-Mares DA, Acevedo-Fernández JJ,
Waksman-Minsky N, Salazar-Aranda R. Evaluation
of antihyperglycemic activity of plants
in northeast mexico. Cell Mol Biol
(Noisy-le-grand). 2021;67(1):212-218.
Anderson AC, Al-Ahmad A, Elamin F, Jonas
D, Mirghani Y, Schilhabel M, Karygianni L,
Hellwig E, Rehman A. Comparison of the
bacterial composition and structure in symptomatic
and asymptomatic endodontic infections
associated with root-filled teeth using
p y r o s e q u e n c i n g . P L o S O n e .
2013;8(12):e84960.
Annemer S, Farah A, Stambouli H, Assouguem
A, Almutairi MH, Sayed AA, Peluso I,
Bouayoun T, Talaat Nouh NA, El Ouali
Lalami A, Ez Zoubi Y. Chemometric Investigation
and Antimicrobial Activity of Salvia
rosmarinus Spenn Essential Oils. Molecules.
2022;27(9):2914.
Bisio A, De Mieri M, Milella L, Schito AM,
Parricchi A, Russo D, Alfei S, Lapillo M, Tuccinardi
T, Hamburger M, De Tommasi N.
Antibacterial and Hypoglycemic Diterpenoids
from Salvia chamaedryoides. J Nat Prod.
2017;80(2):503-514.
Búfalo J, Cantrell CL, Jacob MR, Schrader
KK, Tekwani BL, Kustova TS, Ali A, Boaro
CS. Antimicrobial and Antileishmanial Activities
of Diterpenoids Isolated from the Roots
of Salvia deserta. Planta Med.
2016;82(1-2):131-7.
Chen X, Ding J, Ye YM, Zhang JS. Bioactive
abietane and seco-abietane diterpenoids from
Salvia prionitis. J Nat Prod.
2002;65(7):1016-20.
Córdova-Guerrero I, Aragon-Martinez OH,
Díaz-Rubio L, Franco-Cabrera S,
Serafín-Higuera NA, Pozos-Guillén A,
Soto-Castro TA, Martinez-Morales F, Isiordia-
Espinoza M. Antibacterial and antifungal
activity of Salvia apiana against clinically
important microorganisms. Rev Argent
Microbiol. 2016;48(3):217-221.
Cvetkovikj I, Stefkov G, Acevska J, Stanoeva
JP, Karapandzova M, Stefova M, Dimitrovska A, Kulevanova S. Polyphenolic characterization
and chromatographic methods for fast
assessment of culinary Salvia species from
South East Europe. J Chromatogr A.
2013;1282:38-45.
Fahed L, Stien D, Ouaini N, Eparvier V, El
Beyrouthy M. Chemical Diversity and Antimicrobial
Activity of Salvia multicaulis Vahl
Essential Oils. Chem Biodivers.
2016;13(5):591-5.
Ghorbanpour M, Hatami M, Kariman K,
Abbaszadeh Dahaji P. Phytochemical Variations
and Enhanced Efficiency of Antioxidant
and Antimicrobial Ingredients in Salvia officinalis
as Inoculated with Different Rhizobacteria.
Chem Biodivers. 2016;13(3):319-330.
Gören AC, Topçu G, Oksüz S, Kökdil G,
Voelter W, Ulubelen A. Diterpenoids from
Salvia ceratophylla. Nat Prod Lett.
2002;16(1):47-52.
Hong BY, Lee TK, Lim SM, Chang SW, Park
J, Han SH, Zhu Q, Safavi KE, Fouad AF, Kum
KY. Microbial analysis in primary and
persistent endodontic infections by using
p y r o s e q u e n c i n g . J - E n d o d .
2013;39(9):1136-40.
Iaculli F, Rodríguez-Lozano FJ, Briseño-Marroquín
B, Wolf TG, Spagnuolo G, Rengo S.
Vital Pulp Therapy of Permanent Teeth with
Reversible or Irreversible Pulpitis: An Overview
of the Literature. J Clin Med.
2022;11(14):4016.
Kabouche A, Boutaghane N, Kabouche Z,
Seguin E, Tillequin F, Benlabed K. Components
and antibacterial activity of the roots of
S a l v i a j a m i n i a n a . F i t o t e r a p i a .
2005;76(5):450-2.
Krzyściak W, Jurczak A, Kościelniak D,
Bystrowska B, Skalniak A. The virulence of
Streptococcus mutans and the ability to form
biofilms. Eur J Clin Microbiol Infect Dis.
2014;33(4):499-515.
Kuźma Ł, Rózalski M, Walencka E, Rózalska
B, Wysokińska H. Antimicrobial activity of
diterpenoids from hairy roots of Salvia sclarea
L.: salvipisone as a potential anti-biofilm
agent active against antibiotic resistant Staphylococci.
Phytomedicine. 2007;14(1):31-5.
Kuźma L, Wysokińska H, Różalski M,
Budzyńska A, Więckowska-Szakiel M, Sadowska
B, Paszkiewicz M, Kisiel W, Różalska
B. Antimicrobial and anti-biofilm properties
of new taxodione derivative from hairy roots
of Salvia austriaca. Phytomedicine.
2012;19(14):1285-7.
Lim Ah Tock MJ, Kamatou GPP, Combrinck
S, Sandasi M, Viljoen AM. A chemometric
assessment of essential oil variation of three
Salvia species indigenous to South Africa.
Phytochemistry. 2020;172:112249.
Lucchetti L, Zitti S, Taffetani F. Ethnobotanical
uses in the Ancona district (Marche
region, Central Italy). J Ethnobiol Ethnomed.
2019;15(1):9.
Martel J, Ojcius DM, Ko YF, Chang CJ,
Young JD. Antiaging effects of bioactive
molecules isolated from plants and fungi.
Med Res Rev. 2019;39(5):1515-1552.
Ngo TM, Tran PT, Hoang LS, Lee JH, Min
BS, Kim JA. Diterpenoids isolated from the
root of Salvia miltiorrhiza and their anti-inflammatory
activity. Nat Prod Res.
2021;35(5):726-732.
Rosales-López C, Arnáez-Serrano E, Moreira-
González I, Garro-Monge G, Agüero-
Hernández A, Jiménez-Quesada K, Abdelnour-
Esquivel A, Calvo-Castro L. Investigaciones
en plantas con potencial bioactivo.
Tecnología en Marcha. Especial 2019. 25
Aniversario del Centro de Investigación en
Biotecnología. 2019;32:12-2.
Rouhani A, Javadzadeh A, Tanhaeian A,
Navabi S. A Comparison of Antibacterial
Properties of Tachyplesin, Thanatin, and
Enterocin P on Enterococcus faecalis. Eur
Endod J 2022; 7: 67-72.
Shara M, Stohs SJ. Efficacy and Safety of
White Willow Bark (Salix alba) Extracts.
Phytother Res. 2015;29(8):1112-6.
Tung NH, Nakajima K, Uto T, Hai NT, LongDD, Ohta T,
Oiso S, Kariyazono H, Shoyama
Y. Bioactive Triterpenes from the Root of
Salvia miltiorrhiza Bunge. Phytother Res.
2017;31(9):1457-1460.
Tzanetakis GN, Azcarate-Peril MA, Zachaki S,
Panopoulos P, Kontakiotis EG, Madianos PN,
Divaris K. Comparison of Bacterial Community
Composition of Primary and Persistent Endodontic
Infections Using Pyrosequencing. J
Endod. 2015;41(8):1226-33.
Ulubelen A, Sönmez U, Topcu G, Bozok
Johansson C. An abietane diterpene and two
phenolics from Salvia forskahlei. Phytochemistry.
1996;42(1):145-7.
Ulubelen A, Oksüz S, Topcu G, Gören AC,
Bozok-Johansson C, Celik C, Kökdil G,
Voelter W. A new antibacterial diterpene from
the roots of Salvia caespitosa. Nat Prod Lett.
2001a;15(5):307-14.
Ulubelen A, Oksüz S, Topcu G, Gören AC,
Voelter W. Antibacterial diterpenes from the
roots of Salvia blepharochlaena. J Nat Prod.
2001b;64(4):549-51.
Verweij J, Clavel M, Chevalier B. Paclitaxel
(Taxol) and docetaxel (Taxotere): not simply
two of a kind. Ann Oncol. 1994;5(6):495-505
Villa-Ruano N, Zurita-Vásquez GG, Pacheco-
Hernández Y, Betancourt-Jiménez MG,
Cruz-Durán R, Duque-Bautista H. Anti-lipase
and antioxidant properties of 30 medicinal
plants used in Oaxaca, México. Biol Res.
2013;46(2):153-60.
Wang L, Ma R, Liu C, Liu H, Zhu R, Guo S,
Tang M, Li Y, Niu J, Fu M, Gao S, Zhang D.
Salvia miltiorrhiza: A Potential Red Light to
the Development of Cardiovascular Diseases.
Curr Pharm Des. 2017;23(7):1077-1097.
Weaver BA. How Taxol/paclitaxel kills cancer
cells. Mol Biol Cell. 2014;25(18):2677-2681.
Wicks C, Hudlicky T, Rinner U. Morphine
alkaloids: History, biology, and synthesis.
Alkaloids Chem Biol. 2021;86:145-342.
Will M, Claßen-Bockhoff R. Time to split
Salvia s.l. (Lamiaceae) - New insights from
Old World Salvia phylogeny. Mol Phylogenet
Evol. 2017;109:33-58.
Zupkó I, Hohmann J, Rédei D, Falkay G,
Janicsák G, Máthé I. Antioxidant activity of
leaves of Salvia species in enzyme-dependent
and enzyme-independent systems of lipid
peroxidation and their phenolic constituents.
Planta Med. 2001;67(4):366-8.
Plant-Based Bioactive Molecules in Improving
Health and Preventing Lifestyle Diseases. Int J
Mol Sci. 2021;22(6):2991.
Adame-Miranda SJ, Granados-Guzmán G,
Silva-Mares DA, Acevedo-Fernández JJ,
Waksman-Minsky N, Salazar-Aranda R. Evaluation
of antihyperglycemic activity of plants
in northeast mexico. Cell Mol Biol
(Noisy-le-grand). 2021;67(1):212-218.
Anderson AC, Al-Ahmad A, Elamin F, Jonas
D, Mirghani Y, Schilhabel M, Karygianni L,
Hellwig E, Rehman A. Comparison of the
bacterial composition and structure in symptomatic
and asymptomatic endodontic infections
associated with root-filled teeth using
p y r o s e q u e n c i n g . P L o S O n e .
2013;8(12):e84960.
Annemer S, Farah A, Stambouli H, Assouguem
A, Almutairi MH, Sayed AA, Peluso I,
Bouayoun T, Talaat Nouh NA, El Ouali
Lalami A, Ez Zoubi Y. Chemometric Investigation
and Antimicrobial Activity of Salvia
rosmarinus Spenn Essential Oils. Molecules.
2022;27(9):2914.
Bisio A, De Mieri M, Milella L, Schito AM,
Parricchi A, Russo D, Alfei S, Lapillo M, Tuccinardi
T, Hamburger M, De Tommasi N.
Antibacterial and Hypoglycemic Diterpenoids
from Salvia chamaedryoides. J Nat Prod.
2017;80(2):503-514.
Búfalo J, Cantrell CL, Jacob MR, Schrader
KK, Tekwani BL, Kustova TS, Ali A, Boaro
CS. Antimicrobial and Antileishmanial Activities
of Diterpenoids Isolated from the Roots
of Salvia deserta. Planta Med.
2016;82(1-2):131-7.
Chen X, Ding J, Ye YM, Zhang JS. Bioactive
abietane and seco-abietane diterpenoids from
Salvia prionitis. J Nat Prod.
2002;65(7):1016-20.
Córdova-Guerrero I, Aragon-Martinez OH,
Díaz-Rubio L, Franco-Cabrera S,
Serafín-Higuera NA, Pozos-Guillén A,
Soto-Castro TA, Martinez-Morales F, Isiordia-
Espinoza M. Antibacterial and antifungal
activity of Salvia apiana against clinically
important microorganisms. Rev Argent
Microbiol. 2016;48(3):217-221.
Cvetkovikj I, Stefkov G, Acevska J, Stanoeva
JP, Karapandzova M, Stefova M, Dimitrovska A, Kulevanova S. Polyphenolic characterization
and chromatographic methods for fast
assessment of culinary Salvia species from
South East Europe. J Chromatogr A.
2013;1282:38-45.
Fahed L, Stien D, Ouaini N, Eparvier V, El
Beyrouthy M. Chemical Diversity and Antimicrobial
Activity of Salvia multicaulis Vahl
Essential Oils. Chem Biodivers.
2016;13(5):591-5.
Ghorbanpour M, Hatami M, Kariman K,
Abbaszadeh Dahaji P. Phytochemical Variations
and Enhanced Efficiency of Antioxidant
and Antimicrobial Ingredients in Salvia officinalis
as Inoculated with Different Rhizobacteria.
Chem Biodivers. 2016;13(3):319-330.
Gören AC, Topçu G, Oksüz S, Kökdil G,
Voelter W, Ulubelen A. Diterpenoids from
Salvia ceratophylla. Nat Prod Lett.
2002;16(1):47-52.
Hong BY, Lee TK, Lim SM, Chang SW, Park
J, Han SH, Zhu Q, Safavi KE, Fouad AF, Kum
KY. Microbial analysis in primary and
persistent endodontic infections by using
p y r o s e q u e n c i n g . J - E n d o d .
2013;39(9):1136-40.
Iaculli F, Rodríguez-Lozano FJ, Briseño-Marroquín
B, Wolf TG, Spagnuolo G, Rengo S.
Vital Pulp Therapy of Permanent Teeth with
Reversible or Irreversible Pulpitis: An Overview
of the Literature. J Clin Med.
2022;11(14):4016.
Kabouche A, Boutaghane N, Kabouche Z,
Seguin E, Tillequin F, Benlabed K. Components
and antibacterial activity of the roots of
S a l v i a j a m i n i a n a . F i t o t e r a p i a .
2005;76(5):450-2.
Krzyściak W, Jurczak A, Kościelniak D,
Bystrowska B, Skalniak A. The virulence of
Streptococcus mutans and the ability to form
biofilms. Eur J Clin Microbiol Infect Dis.
2014;33(4):499-515.
Kuźma Ł, Rózalski M, Walencka E, Rózalska
B, Wysokińska H. Antimicrobial activity of
diterpenoids from hairy roots of Salvia sclarea
L.: salvipisone as a potential anti-biofilm
agent active against antibiotic resistant Staphylococci.
Phytomedicine. 2007;14(1):31-5.
Kuźma L, Wysokińska H, Różalski M,
Budzyńska A, Więckowska-Szakiel M, Sadowska
B, Paszkiewicz M, Kisiel W, Różalska
B. Antimicrobial and anti-biofilm properties
of new taxodione derivative from hairy roots
of Salvia austriaca. Phytomedicine.
2012;19(14):1285-7.
Lim Ah Tock MJ, Kamatou GPP, Combrinck
S, Sandasi M, Viljoen AM. A chemometric
assessment of essential oil variation of three
Salvia species indigenous to South Africa.
Phytochemistry. 2020;172:112249.
Lucchetti L, Zitti S, Taffetani F. Ethnobotanical
uses in the Ancona district (Marche
region, Central Italy). J Ethnobiol Ethnomed.
2019;15(1):9.
Martel J, Ojcius DM, Ko YF, Chang CJ,
Young JD. Antiaging effects of bioactive
molecules isolated from plants and fungi.
Med Res Rev. 2019;39(5):1515-1552.
Ngo TM, Tran PT, Hoang LS, Lee JH, Min
BS, Kim JA. Diterpenoids isolated from the
root of Salvia miltiorrhiza and their anti-inflammatory
activity. Nat Prod Res.
2021;35(5):726-732.
Rosales-López C, Arnáez-Serrano E, Moreira-
González I, Garro-Monge G, Agüero-
Hernández A, Jiménez-Quesada K, Abdelnour-
Esquivel A, Calvo-Castro L. Investigaciones
en plantas con potencial bioactivo.
Tecnología en Marcha. Especial 2019. 25
Aniversario del Centro de Investigación en
Biotecnología. 2019;32:12-2.
Rouhani A, Javadzadeh A, Tanhaeian A,
Navabi S. A Comparison of Antibacterial
Properties of Tachyplesin, Thanatin, and
Enterocin P on Enterococcus faecalis. Eur
Endod J 2022; 7: 67-72.
Shara M, Stohs SJ. Efficacy and Safety of
White Willow Bark (Salix alba) Extracts.
Phytother Res. 2015;29(8):1112-6.
Tung NH, Nakajima K, Uto T, Hai NT, LongDD, Ohta T,
Oiso S, Kariyazono H, Shoyama
Y. Bioactive Triterpenes from the Root of
Salvia miltiorrhiza Bunge. Phytother Res.
2017;31(9):1457-1460.
Tzanetakis GN, Azcarate-Peril MA, Zachaki S,
Panopoulos P, Kontakiotis EG, Madianos PN,
Divaris K. Comparison of Bacterial Community
Composition of Primary and Persistent Endodontic
Infections Using Pyrosequencing. J
Endod. 2015;41(8):1226-33.
Ulubelen A, Sönmez U, Topcu G, Bozok
Johansson C. An abietane diterpene and two
phenolics from Salvia forskahlei. Phytochemistry.
1996;42(1):145-7.
Ulubelen A, Oksüz S, Topcu G, Gören AC,
Bozok-Johansson C, Celik C, Kökdil G,
Voelter W. A new antibacterial diterpene from
the roots of Salvia caespitosa. Nat Prod Lett.
2001a;15(5):307-14.
Ulubelen A, Oksüz S, Topcu G, Gören AC,
Voelter W. Antibacterial diterpenes from the
roots of Salvia blepharochlaena. J Nat Prod.
2001b;64(4):549-51.
Verweij J, Clavel M, Chevalier B. Paclitaxel
(Taxol) and docetaxel (Taxotere): not simply
two of a kind. Ann Oncol. 1994;5(6):495-505
Villa-Ruano N, Zurita-Vásquez GG, Pacheco-
Hernández Y, Betancourt-Jiménez MG,
Cruz-Durán R, Duque-Bautista H. Anti-lipase
and antioxidant properties of 30 medicinal
plants used in Oaxaca, México. Biol Res.
2013;46(2):153-60.
Wang L, Ma R, Liu C, Liu H, Zhu R, Guo S,
Tang M, Li Y, Niu J, Fu M, Gao S, Zhang D.
Salvia miltiorrhiza: A Potential Red Light to
the Development of Cardiovascular Diseases.
Curr Pharm Des. 2017;23(7):1077-1097.
Weaver BA. How Taxol/paclitaxel kills cancer
cells. Mol Biol Cell. 2014;25(18):2677-2681.
Wicks C, Hudlicky T, Rinner U. Morphine
alkaloids: History, biology, and synthesis.
Alkaloids Chem Biol. 2021;86:145-342.
Will M, Claßen-Bockhoff R. Time to split
Salvia s.l. (Lamiaceae) - New insights from
Old World Salvia phylogeny. Mol Phylogenet
Evol. 2017;109:33-58.
Zupkó I, Hohmann J, Rédei D, Falkay G,
Janicsák G, Máthé I. Antioxidant activity of
leaves of Salvia species in enzyme-dependent
and enzyme-independent systems of lipid
peroxidation and their phenolic constituents.
Planta Med. 2001;67(4):366-8.
Publicado
2024-03-16
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